Diastereomeric Resolution Yields Highly Potent Inhibitor of SARS-CoV-2 Main ProteaseArticle Published on 2022-10-132022-11-15 Journal: Journal of medicinal chemistry [Category] COVID19(2023년), SARS, 치료제, [키워드] 3CL pro administration antiviral activity Antiviral treatment binding cell types complexes COVID-19 COVID-19 pandemic crystal structure enzyme inactive inhibitor M pro polyprotein potent protease Resolution SARS-CoV-2 translated viral replication Viral RNA yield [DOI] 10.1021/acs.jmedchem.2c01131 PMC 바로가기
Galectin-3 binding protein stimulated IL-6 expression is impeded by antibody intervention in SARS-CoV-2 susceptible cell linesArticle Published on 2022-10-112022-11-15 Journal: Scientific Reports [Category] COVID19(2023년), SARS, 진단, [키워드] Activation affected antibody binding cell line cell types Colon contribute COVID-19 COVID-19 patients described disease epithelial expression galectin-3 global pandemic identify IL-6 IL-6 expression inflammatory cells Intervention lung epithelial marker monoclonal antibody pathophysiology pro-inflammatory cytokine Protein receptor Research SARS-CoV-2 severe cases severe COVID-19 patients severe disease severity stimulate stimulated susceptible cell the cytokine storm therapeutic targets Treatment was reduced [DOI] 10.1038/s41598-022-20852-x PMC 바로가기
Adaptive immunity to SARS-CoV-2 infection: A systematic reviewArticle Published on 2022-10-102022-11-15 Journal: Frontiers in Immunology [Category] COVID19(2023년), SARS, 바이오마커, [키워드] acute respiratory syndrome adaptive Adaptive immunity addressed Alpha Antigen article B cell Biomarker case-control study caused CD4+ T cell CD8+ CD8+ T lymphocytes Cell cell types Characteristics clinical benefits Cohort studies coronavirus coronavirus disease Course COVID-19 Critically ill Critically ill patient cross-sectional CXCL9 Cytokines database disease English Evidence experimental evidence follicular HLA-DR humoral Humoral immunity IL-10 IL-13 IL-15 IL-17 IL-1α IL-2 IL-21 IL-22 IL-23 IL-4 IL-5 IL-7 immune system Immunity immunity. immunological Immunological memory immunopathological in vitro assays individuals inflammatory mediator involved leukocyte Ligand lymphocyte marker mild case Necrosis poor prognosis predominant produced programmed cell death progression Protein Regulatory T cell responsible SARS-CoV-2 SARS-COV-2 infection SciELO secretion severity Spanish subpopulation systematic review T helper T helper cell T lymphocyte T-cell Response Tfh TGF-β Th1 Th17 Th2 the antibody the disease TNF-α Treg tumor growth Vaccine variable [DOI] 10.3389/fimmu.2022.1001198 PMC 바로가기
Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assayArticle Published on 2022-09-262022-11-15 Journal: STAR protocols [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] acute respiratory syndrome approach cell membrane cell types Cell-based Assays Complete coronavirus functional living cell living cells membrane fusion Microbiology Microbiology. MONITOR monitoring pathogenicity Protein protocol reporter SARS-CoV-2 Spike protein Suzuki target cells triggering variants viral entry virus [DOI] 10.1016/j.xpro.2022.101773 PMC 바로가기
SARS-CoV-2 Spike Proteins and Cell-Cell Communication Inhibits TFPI and Induces Thrombogenic Factors in Human Lung Microvascular Endothelial Cells and Neutrophils: Implications for COVID-19 Coagulopathy PathogenesisArticle Published on 2022-09-092022-11-16 Journal: International Journal of Molecular Sciences [Category] SARS, 변종, [키워드] ACE2 acute respiratory distress alveolar angiotensin-converting enzyme 2 binding blocked Blood coagulation Capillaries cell types cellular co-culture Coagulopathy communication Contact COVID-19 COVID-19 patient Delta Disease progression drug DTNB endothelial Endothelial cell endotheliopathy Evidence Express expression extrinsic pathway Factor Factor-V fibrinogen functional groups had no Human human lung human lung endothelial cells humans induce infect inhibited inhibitor interactions investigated leukocyte mechanism Microvascular thrombosis neutrophil Neutrophils Pathogenesis pathway Patient potential mechanisms prevented Protein pulmonary complication Pulmonary endothelium recombinant S-protein SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike proteins SARS-CoV-2 variant SARS-CoV-2 virion secretion severe lung injury significantly increased spike syndrome target cell TFPI therapeutic strategy thrombin thrombomodulin thrombosis tissue Tissue factor. Transcriptional upregulation vascular endothelial cell vessel Wuhan [DOI] 10.3390/ijms231810436 PMC 바로가기
Protein Expression Profile of ACE2 in the Normal and COVID-19-Affected Human BrainArticle Published on 2022-09-022022-11-16 Journal: Journal of Proteome Research [Category] COVID19(2023년), SARS, 유전자 메커니즘, [키워드] ACE2 antibodies antibody Brain caused cell types coronavirus disease COVID-19 COVID-19 disease differential expression Endothelial cell enzyme ependymal cell expression expression pattern highest highlight host cell Human immunohistochemistry mechanism Neurological symptom Neurological symptoms NEUROLOGY normal pandemic Patient patients previous study profile protein expression proteomics receptor Region risk SARS-coronavirus SARS-CoV-2 shown suggested Symptom the patient tissue transcriptomics transcriptomics. upregulation [DOI] 10.1021/acs.jproteome.2c00184 PMC 바로가기
Carbohydrate-binding protein from stinging nettle as fusion inhibitor for SARS-CoV-2 variants of concernArticle Published on 2022-08-302022-11-15 Journal: Frontiers in Cellular and Infection Microbiology [Category] SARS, 변종, 치료제, [키워드] addition Antiviral antiviral activity assays Beta binding block Cell cell cultures cell types cell-cell fusion cells circulating Coronaviruses Deletion Delta demonstrated fusion inhibitor Gamma inhibit inhibited inhibitor interface investigated lectin lung epithelial membrane fusion monomeric mutant N-acetylglucosamine (GlcNAc) N-glycosylation omicron Prevent Protein pseudotyped SARS-CoV-2 remained Replication replication of SARS-CoV-2 S2 subunit SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant Spike protein spike protein. SPR subsequent surface plasmon resonance tested the spike protein UDA Urtica variant variants of concern variants of SARS-CoV-2 Vero E6 cell viral replication viral spike protein virus entry viruses Wuhan-Hu-1 [DOI] 10.3389/fcimb.2022.989534 PMC 바로가기
Bimodal distribution pattern associated with the PCR cycle threshold (Ct) and implications in COVID-19 infectionsPCR 주기 역치(Ct)와 관련된 바이모달 분포 패턴 및 COVID-19 감염에 미치는 영향Article Published on 2022-08-252022-09-11 Journal: Scientific Reports [Category] SARS, 변종, 진단, 치료기술, [키워드] 2019-nCoV age bimodal bimodal distribution cell types Chain Reaction Characteristics clinical sample clinical samples Combination Control COVID-19 COVID-19 infection COVID-19 pandemic Ct values cycle threshold disease control Disease progression dissemination distribution DNA epithelial cells examined explain Gender Genome heterogeneity host response implication Infection infection control Integrated DNA Technologies mucosal notable PCR PCR cycle platform polymerase chain reaction positive prevention ranged real-time RT-PCR respiratory respiratory epithelial cells RT-PCR sample collection SARS-CoV-2 stratified TaqPath ThermoFisher Scientific threshold Transmissibility Transmission Viral Viral load viral replication viral variant Viral variants [DOI] 10.1038/s41598-022-18735-2 PMC 바로가기 [Article Type] Article
Multiomic analysis reveals cell-type-specific molecular determinants of COVID-19 severity다중체 분석은 COVID-19 중증도의 세포 유형별 분자 결정 요인을 나타냅니다.Article Published on 2022-08-172022-10-30 Journal: Cell Systems [Category] COVID-19, MERS, 바이오마커, 변종, 유전자 메커니즘, [키워드] Analysis CD56 Cell cell types COVID-19 COVID-19 severity cytokine determinant determinants dysfunction enrichment functions gene discovery Genes Genetic genetic risk Genetic variation genome-wide association Genome-wide association study genomic GWAS healthy human lung innate immune response Intervention limit machine learning mendelian randomization molecular natural killer network analysis NK cell NK cells NK-cell Pathogenesis potential therapeutic targets provide rare variant analysis reveal risk severe COVID-19 severe disease single-cell single-cell multiomic profiling. Support T cells these cell These cells upstream variant [DOI] 10.1016/j.cels.2022.05.007 PMC 바로가기 [Article Type] Article
Modeling SARS-CoV-2 and influenza infections and antiviral treatments in human lung epithelial tissue equivalents인간 폐 상피 조직 동등물에서 SARS-CoV-2 및 인플루엔자 감염 및 항바이러스 치료 모델링Article Published on 2022-08-122022-09-11 Journal: Communications Biology [Category] SARS, 바이오마커, 진단, 치료기술, 치료제, [키워드] alveolar Analysis antiviral drug Antiviral treatment antiviral treatments Biomarker cell type cell types cellular chemokine class Critical CXCL10 cytokine Cytokine storm develop emerging viruses epithelial exhibiting human lung IAV IFN response IL-10 IL-6 in vitro in vivo include infect Infection infections Influenza influenza A influenza A virus influenza infection MHC MHC class I modeling Pathogens peaking pharmacological platform Replication robust SARS-CoV-2 SARS-COV-2 infection severe SARS severe SARS-CoV-2 Single-cell RNA sequencing throughput tissue tissues tracheobronchial Validity Viral viral infection virus viruses [DOI] 10.1038/s42003-022-03753-7 PMC 바로가기 [Article Type] Article